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Abstract Abstract: Resveratrol is a member of stilbenoids with the capcity of disease-resistantance for plants and health benefit for human. Carrot is one of the most important vegetables rich in β-carotene. To further promote carrot’s heath care value by expression of foreign resveratrol, we conducted the genetic transformation of RS to carrot. the cDNA of grape resveratrol synthase gene was isolated from a cDNA library of grape and its codon for amino acids was optimized for efficient expression in carrot. With a cDNA of RS, the transformation vecter was constructed by inserting the cDNA into a transformation vector pCB, whose HPT was eliminated. pCB and pCB-RS-hyg- were co-transformed into carrot via a efficiently Agrobacterium mediaed transformation method and transgenic plants were acquired. By screening in the T0, T1 and T2 generation of transgenic plants, 2 plants with high content of resveratrol were found, Which laid a very important base for further use in health care food exploitation and study of resveratrol metabolic pathway.
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Received: 13 April 2007
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