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Abstract Dr lin who works in the biotechnology research institute has isolated and cloned the structural gene aroAG2 coding for EPSP Synthase sequence from Pseudomonas fluorescens G2. The aroAG2M gene was artificially synthesized according to codon usage preferred by dicotyledonous plants.Impactvector1.4 with the chloroplast signal peptide is one of high performing vectors for expression in plant. The aroAG2M gene was placed under the Rubisco promoter and then a plant expression vector was constructed. Transgenic tobacco were obtained by Agrobacterium-mediated transformation．PCR and Southern analysis of transgenic tabacco plants indicated that the exogenous genes were integrated into the genomes of the transgenic plants,and in transgenic plants herbicide-resistance to glyphosate reaches 4‰.The vector was used for cotton transformation by Pollen Tube Pathway Transgenic Technique. PCR and Southern analysis of transgenic cotton plants showed the target gene was integrated into the genome. To date, T1 generation of transgenic plants have been obtained. Transgenic plants are resistant to glyphosate herbicide in field．
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Received: 01 April 2007
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