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Abstract Abstract: β-1.4-N-acetylgalactosaminyl-transferase(Galnact2) is responsible for the first committed step in the synthesis of mucin-type O-glycans on protein substrates。In this study, primers flanking the galnact2 c.363 C>G mutation were designed according to the known chicken galnact-2 DNA sequence. This polymorphism was genotyped in 276 animals from six chicken breeds including White Recessive Rock, Xinghua, Taihe Silk, Baier Huang, Kangle, Nancheng Black, and in a F2 full sib resource population by crossing White Recessive Rock with Xinghua using PCR-XhoI-RFLP. The results showed that：(1) The genetic polymorphism of the Galnact2 c.363C>G mutation was abundant; (2) This polymorphism was significantly associated with body weight at all ages measured. Individuals with the GG genotype had heavier body weight (BW) at the ages of 1, 21, 28, 35, 49, 56, 63, 77 and 84 days than those with the CC genotype (p<0.01). GC individuals had heavier BW at the ages of 1, 21, 56, 63 days (p<0.01), and 35, 49, 77 and 84 days (p<0.05) than CC individuals. There was no significant difference between GC and GG animals for BW at all ages measured; (3) This polymorphism was not significantly associated with fatness traits including fat pad under the skin, abdominal fat weight, width of fat band, crude fat content in breast muscle and crude fat content in leg muscle.
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Received: 20 April 2007
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