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| Chromosomal Assignment, Cloning and Expression of Porcine NDUFS7 Gene in skeletal muscle |
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Abstract Abstract: The chromosomal assignment of porcine NDUFS7 gene was carried out in this experiment using radiation hybrid panel. The complete coding sequence of porcine NDUFS7 gene was obtained by the reverse transcriptase-polymerase chain reaction (RT-PCR), and the prediction of its protein structure and potential function was carried out by regarding tools. The spatial and temporal expression profile of NDUFS7 gene was analyzed by semiquantitative-RT-PCR in porcine 11 tissues and skeletal muscle from pig fetuses at 33days post coitus (dpc), 65dpc, 90dpc and grown pigs, respectively. The results showed that NDUFS7 gene was assigned on the SSC２q21-q24 and linked with marker SW395. The coding sequence of NDUFS7 is 648 nt, coding for 215 amino acids. The predicted results indicated that the porcine NDUFS7 gene has a conservative NuoB domain and 3 phosphorylation sites. Meanwhile, the NDUFS7 gene showed a different expressive level in different porcine tissues, while the expressive level of NDUFS7 in prenatal skeletal muscle at 65dpc and 90dpc was relatively higher than that of in skeletal muscle from the fetuses at 33dpc and the grown pigs.
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Received: 09 January 2007
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