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Abstract To obtain the higher expression efficiency of producing recombinant protein in Escherichia coli, the prokaryotic expressing vector pET-S1, which contained whole antigenic sites of S gene from swine transmissible gastroenteritis coronavirus (TGEV), was constructed and transformed into E.coli strain BL21 (DE3). The target protein was expressed by the IPTG inducing expression system and auto-induction expression system, respectively. The highest amount of target protein accounted for 25.9% and 45.1% of total bacteria protein, respectively， indicating that the expression efficiency was significantly improved by auto-induction expression system.
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Received: 07 January 2008
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