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| Cloning and Fusion Expression of the Gene MalQ from Streptomyces TE66 |
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Abstract The gene MalQ was amplified from Streptomyces coelicolor by PCR, then the gene MalQ was cloned into pTrc-CKS and sequenced. MalQ sequence was analyzed by BLAST and it displayed high similarity(97%) to gene MalQ of Streptomyces coelicolor reported in GenBank. The recombined plasmid pTrc-CKS-MalQ was transformated into E.coli Top 10F’and induced by IPTG.. Gene MalQ and CKS fusion protein(molecular weight：about 106kDa) could detected by SDS-PAGE gel electrophoresis. The crude enzyme was demonstrated having 4- -glucanotransferase activity with TLC.
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Received: 18 June 2007
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