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Abstract Objective To explore the correlation between the polymorphism of Single nucleotide polymorphisms(SNPs) of Resistin and the reguration of fat diposition. Methods Screening formutations in the entire coding region for the Resisin gene were Performed by the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in Lantang pig、Luchuan pig、Lingao pig、Landrace、Large white and duroc pigs. Results Detected results of genotype demonstrate that no polymorphism in exon-1 and exon-3,two genotypes in exon-2(CC、GG),three genotypes in promoter region(CC、TT、CT),six genotypes in intron-1(EE、FF、GG、EF、EG、FG) and intron-2(LL、MM、NN、LM、LN、MN). Variance analysis revealed that Resistin gene had a high linkage with their growth and fatness traits.Conclusion There are SNPs in resistin gene in pigs ,and have association of these SNPs with fat diposition and obesity in pigs.
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Received: 26 July 2007
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