|
|
|
| Study on the expression analysis of IGF2 gene in fetus skeletal muscle from Tongcheng and Landrace pigs |
| Yong Li Hong Deng Shulin Yang Yulian Mou Mingxing Chu Yuehui Ma Kui Li |
| |
|
|
|
|
Abstract IGF2 gene plays an important role in the development of skeletal muscle. In order to investigate the effect of IGF2 on the regulation of development of prenatal skeletal muscle in pigs, the SYBR-Green real-time PCR method was established and used for expression analysis of IGF2 in the prenatal skeletal muscles from Tongcheng and Landrace pigs at 33, 65 and 90 days post coitus (dpc). The results indicated that the real-time PCR method established in this study was effective for the expression analysis of IGF2 gene. IGF2 had the highest expression at mRNA level in skeletal muscle at 65 dpc among selected stages and exhibited the wave expression pattern in two breeds. Interestingly, the IGF2 expression was higher in Tongcheng than that in Landrace pigs at all three prenatal stages studied, but the reason is still unknown.
|
|
Received: 16 August 2007
|
|
Corresponding Authors:
Kui Li
|
|
|
|
| [1] |
ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)[J]. 农业生物技术学报, 2019, 27(8): 1382-1391. |
|
|
|
|
