|
Abstract Balb/C mice（Mus muscalus） were immunized with bovine serum albumin and sulfamethoxazole （BSA-SMZ）, hybridoma lines that secrete monoclonal antibody against sulfamethoxazole（SMZ mAb） were filtered with cell fusion and the immunological traits of SMZmAb were characterized. A stript for detection of SMZ（SMZ-strip） was developed with SMZmAb and its traits were tested. The results showed that three hybridoma lines were filtered and the best one was 3G6 which secreted SMZ mAb with indirect ELISA titers of 1∶8.1 ×102 in supernatant and 1∶6.4×105 in ascites. SMZ mAb had a high affinity constant（Ka） with 3.07×109 L/mol, a good sensitivity with an IC50 of 12.4 μg/L to SMZ, 2.84% cross-reactivity to sulfadiazine and little or no cress-reactivity to other compounds. Its detection limit was 6 μg/L and its sensitivity was as same as that of competitive ELISA-kit and its coincidence rate was 100% compared with ELISA-kit. The SMZ-strip possesses rapidity, sensitivity, specificity and briefness and is proved to be used for the rapid test of SMZ residues.
|
|
Received: 18 June 2008
|
|
|
|
