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Abstract Plant expression vector pBI121/Ω4A/aac was constructed by cloning of aac gene which can quench the quorum-sensing of Ralstonia solanacearum. The aac gene was transferred into tobacco mediated by Agrobacterium tumefaciens. Under Kanamycin selection pressure, fifty-seven resistant lines were obtained. PCR, RT-PCR analysis indicated that aac gene had been integrated in tobacco genome and translated. Inoculation testing showed that the disease index of transgenic tobacco was reduced by 51 in greenhouse after 16 days of inoculation, 56.5 percentage points down from the contrast.
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Received: 23 January 2008
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