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Abstract The pokeweed antiviral protein (PAP) gene was transferred into the cotyledons of mustard (Brassica Juncea Coss.) mediated by Agrobacterium tumefaciens. Using PCR technology to analyse 40 anti-kanamycin plants, 29 positive plants were obtained. The result of Southern blot analysis was that there were single copy or double copies of transgene in the transgenic plants. The result of Northern blot analysis was that the PAP gene was normally expressed in transgenic plants. The results of transgenic plants inoculated with virus TuMV showed that the transgenic plants were more resistant to TuMV than non-transgenic plants.
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Received: 21 November 2007
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