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| cDNA Cloning and Expression Analysis of Maize Protein Kinase Gene ZmCIPK1 in response to abiotic stress |
| BIAN Ming-Di WU Zhong-Yi ZHAO Jiu-Ran |
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Abstract Abstract: Protein kinase plays an important role in stress signal transduction in plant. One full-length cDNA encoding a CIPKs homologue was isolated from maize through in silico cloning and named as ZmCIPK1. The predicted ZmCIPK1 protein has 465 amino acids with an estimated molecular mass of 51.98 KD and an isoelectric point of 6.92. The putative kinase catalytic domain of ZmCIPK1 contains the 11 subdomains that are typical in protein kinase. The conserved 24-amino acid motif within the C-terminal non-kinase region of the CIPKs is also found in ZmCIPK1. RT-PCR analysis indicated that ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature in young leaves and kept high level during 24 hours. These results demonstrate that ZmCIPK1 is a novel CIPK gene and responds to multiple abiotic signals in maize.
key words: maize; CIPK; abiotic stress
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Received: 03 January 2008
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Corresponding Authors:
BIAN Ming-Di
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