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| Isolation, Mapping and Application of a Repetitive DNA Sequence in Wheat (Triticum aestivum) A, B genomes |
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Abstract In this study, SSR analysis was performed on five Secale species, four Triticum species, and a Triticale with branch-like using 102 pairs of microsatellite primers. A 387bp specific DNA fragment FZ387 (Accession No. EF179137), was obtained from Triticale with branched-like by primer Xgwm614; however no amplification result revealed in Secale. The result of sequence comparison revealed this sequence had 94% and 95% similarity with a part of Gypsy Ty3-LTR-retrotransposon fatima in T. monoccocum (AY485644) and T. turgidum（AY494981）, respectively. Based on the conservative region of this sequence, a pair of specific PCR primers, FaF and FaR, was designed. The result of amplification by Xgwm614F and FaR revealed that a specific DNA band of about 350 bp (designation as A350) was obtained from species containing A chromosomes; however, this segment was not appeared in materials not contain A chromosome. Chromosome map was performed on Landon Chinese Spring substitution lines and the result suggested that this segment was located on both long and short arms of all A chromosomes. Also the result of amplification by FaF and Xgwm614R appeared that a specific DNA band with about 350bp (designation as AB350) was obtained from materials containing A or/and B chromosomes; nevertheless this band was not revealed in materials not contain A and B genomes. Using the two pairs of primers, the result of amplification on relative species of Triticum revealed only A350 and AB350 in CS. The result of sequence comparison and variation of SSR primers binding regions of FZ387 indicated that significant diversity may existed in the internal sequence of this fatima element between genus and similarities within genus. Meanwhile, A350 and AB350 could be used as molecular markers for the detection of A and AB chromosomes.
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Received: 27 March 2008
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