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| Detection of SNPs of Bovine Angiopoietin-like protein 4 Gene(ANGPTL4) Coding Region and Its Association with Growth Traits in Nanyang Cattle |
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Abstract Angiopoietin-like protein 4(ANGPTL4) is a secretory protein associated with lipid metabolism. In order to study the function of ANGPTL4 gene in the regulation fat and energy metabolism, in this study, 789 blood samples were collected from 4 bovine populations Nanyang, Jiaxian Red, Luxi and Qinchuan cattles to extract genomic DNA, and SNPs of ANGPTL4 gene were detected by DNA sequencing. Then PCR-RFLP and association analysis between detected SNPs locus and growth traits were carried out. The results indicated that there were missense mutation(1422T>C) and synonymous mutation(4899C>T) in 3rd and 5th exon of ANGPTL4 gene (NC_007305.4), respectively. The association of polymorphisms revealed that the exon 3 and 5 of ANGPTL4 gene had significant correlation with average daily gain of 12-month-old Nanyang cattle(P<0.05); The 3rd exon of ANGPTL4 also had significant correlation with body weight of 12-month-old Nan Yang cattle(P<0.05). The results suggest that these loci may be considered as molecular marker in the marker-assisted selection program for growth traits in Nanyang cattle.
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Received: 14 March 2011
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