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| Cloning and Function Confirmation of Promoters from the Active ω-seclin Genes in a Wheat 1B/1R Translocation Line |
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Abstract Wheat 1B/1R translocation lines have been widely used in China because of their high yield and wide adaptability. However they have a common defect that is bad grain processing quality. ω-secalin is believed to be an important factor for the bad quality. It is a good strategy to silence these ω-secalin genes through RNAi. Using ω-secalin gene's promoter to construct the RNAi expression vector can make the introduced gene to express in needed tissue and needed time and thus raise the effect of molecular breeding. In order to get a active promoter from ω-seclin genes, one pair of specific primers was designed. Promoter cloning was carried out with Lankao 906 as the material, a wheat (Triticum aestivum) 1B/1R translocation line. Five promoters A9-1, H2-1, H7-1, C11 and F11-1 corresponding to three active ω-seclin genes were obtained. Two promoters A9-1 and F11-1 corresponding to two active ω-seclin genes were chosen to make expression vectors with GUS as the marker gene. Transient expression analysis of the GUS gene was made by bombarding young wheat seeds and one promoter F11-1's activity was confirmed. The results provide the basic data for the construction of RNA interfering expression vector driven by the ω secalin gene itself promotor.
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Received: 07 August 2010
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