Abstract OsPHD1 is a member of the plant homedomain(PHD)-finger transcription factor family in rice(Oryza sative). Expression of OsPHD1 is induced under various stresses, including drought, high salinity and cold treatments.By using Agrobacterium-mediated method, OsPHD1 was successfully introduced into rice cultivar Zhonghua 11(Oryza sativa ssp. japonica) and T1 generation of transgenic rice was achieved. The results indicated that overexpression of OsPHD1 in rice could significantly improve more than 43.3%, 60 % and 25% respectively in drought(70%~95% relative water content), high saalinity(200 mmol/L) and cold tolerance(4~8℃) of transgenic plants, and this phenotype was well confirmed in transgenic plants of T2 generation, meanwhile, transgenic plant was almost not different to control in some field characters. Subcellular localization assay of OsPHD1 by using transient expression of OsPHD1::GFP fusion protein in onion(Allium cepa) cells revealed nuclear localization of OsPHD1 protein. Results of qRT-PCR suggested that OsPHD1 protein regulated the anti-reversibility of plants by regulating the stress response of gene expression. Results imaged that OsPHD1 gene has an important application prospect for the anti-reversibility of palnt breeding.
