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Abstract In order to clarify the genetic presentation of the Lycopene-β-cyclase(LYC-b) antisense gene in the progeny of transgenic tomato, the genetic stability of transgenic LYC-b antisense gene of T1 and T2 progenies of tomato(Lycopersicum esculentum) 2 varieties were studied, based on the previous getting transgenic LYC-b antisense gene plant, through detection of transgenicLYC-b antisense gene, measurement of fruit lycopene and soluble solid contents, and morphological and biological characteristics. The results showed that LYC-b antisense gene was exsisted and expressed in T2 plants, which was from positive T1 plant. This means target gene can be stably inherited from one generation to another. There were some differences between cultivars, generations, and plants of the same generation in the effect of LYC-b antisense gene on fruit lycopene content. The fruit lycopene content of T1 transgenetic plants increased by 24%~72% compare to the control while T1 transgenetic plants increased by 24.5%~105.3%. In addition, the flower number per infloresence was less thanthat of the negative control; the soluble solids content of transgenic tomato plants was different between generations and plants; one of T2 generation plants mutated from unlimited growth type to limited one. The results provides some basic data for extending the stable phenotype of transgenic tomato strains in production.
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Received: 09 March 2011
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