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Abstract Transcription factor nanog and oct-4 are the fundamental features of pluripotent Stem Cell, which are the key factors to keep their pluripotency and self-renew ability. We transplanted 10 fibroblast cells(BEF422) into bovine oocytes at GⅤ and MⅡstages respectively, and cultured the oocytes for 24 h in vitro, than we used the RT-PCR and immunofluorescence technique to check the expression and location of nanog and oct-4 in the injected oocytes. The results showed that nanog and oct-4 were all expressed in the injected oocytes and located on transplanted cells nuclear. However, they weren't detected in fibroblast cells and oocytes control group; This result demonstrated that GⅤ and MⅡ stage oocytes both can activate the transcription factor nanog and oct-4 expression to reprogram the fibroblast cells. This study will lay a foundation for further verifying the reprogramme mechanism and related material on donor cells and oocytes.
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Received: 22 November 2010
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LI Zong-Shuai, SHEN Pei-Lei, LIU Lei-Lei, YANG Yang, LI Hai-Jiang, ZHANG Quan-Wei,GONG Ji-Shang, ZHAO Xing-Xu, ZHANG Yong. Obtained Transfection Cell Line for iPSCs Isolation from Bactrian Camels (Camelus bactrianus) via CRISPR/Cas9[J]. 农业生物技术学报, 2019, 27(1): 180-190. |
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