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| The Melanocortin-4 Receptor Gene (MC4R) Expression Detected By SYBR GreenⅠReal-time Quantitative PCR in Landes Geese before and after Overfeeding |
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Abstract The expression characterization of MC4R gene before and after overfeeding plays an important role in fat metabolism of Lades geese. SYBR GreenⅠReal-time quantitative PCR was developed to analyze the expression characterization of MC4R gene in 13 tissues. PCR results showed that both MC4R and β-actin were expressed in all 13 tissues before and after overfeeding of Landes Goose(Casuarius casuarius). SYBR GreenⅠReal-time quantitative PCR results showed that the relative expression of MC4R in kidney, intestine and heart after overfeeding was lower than those before overfeeding, their 2-ΔΔCt values were 0.41±1.80, 0.65±5.75 and 0.72±1.22, respectively. But the relative expressions of MC4R in other tissues were all higher after overfeeding, their 2-ΔΔCT values from high to low were 22.78±1.00 (brain), 9.08±2.80 (liver), 5.28±1.83 (lung), 3.78±3.01 (pancreas), 3.07±3.64(leg muscle), 2.90±0.97 (stomach), 2.34±1.66 (subcutaneous adipose), 2.18±3.01 (abdominal adipose), 2.07±0.37 (chest muscle) and 2.01±1.75 (pleen). We conclude that the results are consistented with the mechanism of MC4R and can provide indirect basis for researches on animal production and human obesity.
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Received: 11 November 2010
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