|
Abstract To probe the infrageneric phylogenetic relationship of Lilium, 19 sequence-related amplified polymorphism (SRAP) primer combinations were chosen to analyze genetic diversity of 23 wild Lilium species, and NTSYS-pc2.1 and POPGENE1.32 were used for data analysis. Result showed that a total of 236 clear DNA bands were amplified, 233 of which were polymorphic, the proportion was 98.72% with average 12.3 polymorphic loci per SRAP primer combination. The genetic similarities ranged from 0.3750 to 0.7679, and the genetic distance ranged from 0.2642 to 0.9808. Twenty three species were classified into four clustering groups by UPGMA. L. regale and L. leucanthum were the mostly closely related systems, whereas L. brownii and L. amabile were the mostly distantly related systems. Materials with similar morphology and from the same source mostly clustered together, and gene exchanges existed between Sect. Lophophorum and Sect. Sinomartagon. The clustering results were basically consistent with morphology classification. The results indicated that SRAP markers are suited to study genetic diversity and phylogenetic relationship of Lilium.
|
|
Received: 22 December 2010
|
|
|
|
