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| Molecular Epidemiological Investigation of Porcine circovirus type 2(PCV2) in Zhejiang Province and Surrounding Areas |
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Abstract In order to investigate the molecular evolutionary characteristics of Porcine circovirus type 2 (PCV2) in Zhejiang and surrounding areas. One hundred and eighty field samples with mixed tissues(spleen, lung and lymph node) from suspect PCV2-infected pigs during 2007~2012 were detected for the PCV2 by the established PCR method, and T-A clone and sequencing were used to get the complete genome of PCV2. Seventy-four cases out of 180 diseased pigs from these herds were PCV2 positive. PCV2-positve rate between 2007 and 2012 (41.7%, 52.0%, 44.1%, 37.0%, 40.5% and 33.3%, respectively) remained in the same level that during 2004~2006. Forty-five PCV2 complete genomes belonged to subgroups 1A (23.9%), 1B (21.7%), 1C (47.8%) and Group 2 (0%), respectively. Group 1 was still the dominant genotype of PCV2 in Zhejiang and surrounding areas. However, as the prevalence of PCV2 in this region, virus belonged to subgroup 1C appeared, increased and gradually dominated. The Cap variability of PCV2 isolates was analyzed in this study, and the result contributed to better grasp the homology of the strains in the detected area.
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Received: 02 August 2012
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