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| Progress of Chromosome Walking the Unknown Promoter Sequences Flanked by a Known Segment |
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Abstract Promoter is a key cis-acting element to regulate gene expression, which is also an important element of the expression vector in genetic engineering. Understanding the structure and function of promoters contributes to constructing vector and controlling the gene expression, further we can achieve the goal that induces protein expression selectively. Therefore introducing a proper method to clone the promoter presents significance and wide application prospects. Since the invention of polymerase chain reaction (PCR) technique, several PCR-based chromosome walking methods have been developed to amplify unknown promoter sequences flanked by a known segment. In this review, we introduce the PCR-based methodologies for the rapid acquisition of unknown promoter, including Inverse PCR, Ligation-mediated PCR, Semi-random primer PCR. These principles and research progresses of each approach are discussed.
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Received: 30 August 2012
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Corresponding Authors:
Xing-Yong YANG
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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