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Abstract Given great biomass yield, high composition of lignocellulose, high use efficiency of feedstock, low cost, wide adaptability, Miscanthus has great potential as an excellent bioenergy crop. M. sinensis is one of the species. This study established an efficient system by using the matured seeds’ embryo and hypocotyl as explants. Significant variations were determined for the efficiency of embryogenic callus production, regeneration, tillering, cellulose and hemicellulose contents, lignin content, cellulose and hemicellulose degradation efficiency in a diverse of M. sinensis genotypes. Additionally, rooting was found during the subculture in all genotypes, which inhibited the regeneration. No significant correlation was detected between the efficiency of embryogenic callus production, regeneration and the lignocelluloses component and their degradation efficiency. For its agronomic traits, lignocelluloses feature and tissue culture result, the genotype, PMS167, was considered as model feedstock of cellulose ethanol. The study is informative for the genetic improvement and efficient micropropagation for Miscanthus.
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Received: 24 April 2013
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