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| Construction and expression of red fluorescent protein reporter gene vector pBacA4DsRed1 |
| Mei-Rong Zhang Yu-Lin Chen Han-Fu Xu Chun Liu Qing-You Xia Ping Zhao |
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Abstract By using of three basic elements, actin4 promoter of Bombyx mori, red fluorescent protein and the identified sequence of polyadenylation acid in SV40, the pBacA4Red1 transgene vector was constructed successfully. Red fluorescence was observed in the three-day preblastodermic eggs after the reconstructed piggyBac expression vector was microinjected. This indicated that the expression vector was correctly constructed and can express in the silkworm eggs. As the green fluorescent protein(GFP), the RFP can also be used as the reporter gene to research the functional gene of silkworm.
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Received: 23 May 2006
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Corresponding Authors:
Mei-Rong Zhang
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