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| Primer Design for a Prokaryotic Differential Display RT- PCR |
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Abstract Due to the lack of polyadenylation in prokaryotic mRNA, the primer design of prokaryotic DDRT-PCR is different from eukaryocyte. Oligo(dT) primers are effectively replaced with primers designed according to highly iterated palindrome in complete genome. The primer design method can not only overcome shortcoming in prokaryotic mRNA to maximatily amplify whole cDNA, but aslo enhance the RNA finger-printing reproducibility and weaken false positive.It supples novel thought for prokaryotic differential display RT- PCR.
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Received: 01 August 2006
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