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| A preliminary research on application of Real-time Fluorescent PCR assay to identify three-line hybrid rice |
| Cheng-Yi CHARLES Shui-Fang ZHU |
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Abstract The research and development of the hybrid rice had paid great contribution to the development of the national economy, and had been popularized in the Southeast asia. How to build up a set of fast, accurate ture-false and purity test system of hybrid rice has been technique hard nut to crack which perplexed quarantine researchers for long time. The real-time fluorescent PCR technique is a newly arisen detect technique in recent years. Using PCR amplification, The reliability of DNA fragment R2-630 was confirmed which related to male sterity reported in 1995.Based on this fragment, one TaqMan probe and one pair of primers were designed, which can detect 17 hybrid rice samples. The result shows: this probe can with peculiar to amplify, hybrid rice and sterile lines peculiarly, and can be used in the purity identification basically. Thus the first step of one time- saving, accurate and quick real-time fluorescent PCR test system was built up.
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Received: 18 May 2006
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Corresponding Authors:
Cheng-Yi CHARLES
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