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Abstract Twenty-five simple sequence repeat (SSR) markers were isolated and characterized in Castanea mollissima from the cultivar Yanhong. For the identification of SSR loci, primer were designed on each side flanking the repeat region and they were initially tested on 6 chestnut samples using chemiluminesence detection. Eighteen loci where shown to be polymorphic and the number of alleles detected per locus varied from 2 to 6. Twelve loci were chosen for the analysis of 24 cultivars grown in North Chinese using the semi-automatic system ABI PRISM 377. These 12 markers showed a high level of genetic polymorphism with a total of 75 alleles; the number of alleles ranged from 4 to 10 per locus, with an average level of 6.3. The mean expected and observed heterozygosity were 0.743 (range: 0.680－0.845) and 0.829 (range: 0.730－0.930) respectively. The estimated frequence of null alleles showed a positive value for 3 loci, but except for 1 locus, the values were very low. The total value for the probability of identity was 7.01×10-11. Paternity exclusion probability was very high (0.999), sufficiently high to study pollen flow.
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Received: 20 June 2006
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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