Abstract According to the two distal and conserved regions of known α-gliadin genes, gene-specific primers for α-gliadin were designed to amplify the full gene coding regions. The amplifled fragments were cloned and sequenced. Three clones with the GenBank accession No. DQ417343, DQ417344 and DQ417345 were screened out, among which, the coding regions of DQ417343 and DQ417345 are 942 and 921bp, enloding the proleins with 313 and 306 amino acid residues, respectively; DQ417344 is a pseudogene by the definition of two in-frame stop codons. Multi-alignment analysis indicated that these sequences had higher similarity with other α-gliadin genes, and the differences mainly existed in the N-terminal repetitive domain and two polyglutamine regions.
