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| Molecular Cloning and Bioinformatic Analysis of Pollen-specific Polygalacturonase, BoMF9 from Brassica oleracea |
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Abstract BoMF9 (BoMF9c and BoMF9l) gene, homologous to the BcMF9 gene encoding pollen-specific polygalacturonase of Chinese cabbage，were cloned from Brassica oleracea var. capitata and B. oleracea var. alboglabra by PCR amplification. Sequence analysis and phylogenetic analysis revealed that the deduced amino acids sequences of BoMF9c and BoMF9l contained characteristic sequence of polygalacturonase expressed in pollen. RT-PCR analysis again showed that BoMF9c and BoMF9l were expressed in flower buds but not other tissues. These two genes may act as polygalacturonases involving in pollen development.
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Received: 06 September 2006
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