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Abstract Based on the consensus of the S-adenosylmethionine synthetase(SAM) and the sequence of the Bacillus thuringiensis genome, we designed a pair of primer for the S-adenosylmethionine synthetase gene of the Bacillus sphaericus.The fragment of S-adenosylmethionine synthetase gene of the Bacillus sphaericus was amplified by PCR. Sequence analysis was done after the fragment was inserted into the the expression vector pGEX-6P-1 to construct the plasmid pGEX-SAM. The results show that the homologous of the fragment with the SAM synthetase gene from E. coli, Bacillus subtilis, Saccharomyces cerevisiae, rat and Homo sapiens is 63.9%，75.4%，58.8%，59.1%，55.7% respectively. For the difference between the fragment and the reported SAM synthetase gene, we expressed the gene in E.coli DH5α and the purified 43KD proterin was acquired. The activity of catalyzing the formation of SAM was identified by determining the produced SAM through High Performance Liquid Chromatography.
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Received: 08 June 2006
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