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Abstract MxA was a new kind of antivirus protein that was induced by IFNα/β.It’s very useful and necessary to study for the mankind. We induced CEF with IFNβand Poly:IC in order to get MxA gene. Then we abstracted total RNA from the CEF. A pair of primers was designed to carry through RT-PCR. The PCR product was sequenced and then they were inserted into a expression plasmid vector. The full-length recombination MxA protein was got by induced E.coli .Then we puried and disposed the product in order to get the active recombination protein.We can know the density of the MxA was 10mg/ml by analyse. The active experiment done in and out of the body proved that the recombination MxA protein had biological activity of interferon virus .We have enough reasons to believe that the recombination MxA protein will has a good and useful prosepect.
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Received: 27 March 2006
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