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| Cloning and vector construction of ovule phospholipase C gene TfPLC1 promoter in Torenia fournieri |
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Abstract Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively. After sequenced, blastn and combined, a TfPLC1 promoter of 1432bp was gained. TATA box and CAAT box-like elements were found in the sequence, and TA domain was rich in the far upstream of the promoter. And also some endosperm-specific elements were found in the sequence. PBI121 and the promoter were double digested to construct plant expression vectors PPP1326 and PPP700 by 5’ deletion. Then the vectors were transformed to Torenia fournieri to validate the function of the promoter.
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Received: 01 June 2006
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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