|
Abstract After seven generations of self-crossing assisted by GUS detection, 26 homozygous transgenic rice lines were obtained. The effects of the transgene on the iron content, agronomic traits, rice quality and stress tolerance were studied taking Xiushui 11 as the control. The results indicated that the average iron contents of the transgenic rice in the milled grain was 10.37 ug/g, among all the homozygous lines, there are only four lines (Fer34, Fer36, Fer39 and Fer65) whose iron contents were significantly
higher than that of the control rice (6.46ug/g); And significant differences in main agronomic traits such as days from sowing to heading, plant height, main panicle Length, total grains of main panicle, seed setting rate, 1000 -grain –weight, yield per plant were found among a few of homozygous lines, except flag leaf length and number of panicles per plant. It showed that inserting foreign gene（Fer）had a slight impact on the main agronomic traits, but the transgene had no negative effects. The homozygous rice lines quality such as the rate of brown rice, milled rice, full milled rice, grain length, grain width, and width ratio were all similar to the control, whereas, variations occurred in chalkiness, translucency, alkali spreading value, gel consistency, amylose content among partial homozygous lines, and did not show obvious regularity. The damage of the homozygous lines treated with low and high temperatures were not significantly correlated with the Fe contents, and the resistance to rice blast, bacterial blight and brown planthopper were enhanced with the advanced of Fe content. Furthermore, the possible causes of the biological characteristics variation among transgenic rice and the effects of foreign gene were discussed.
|
|
Received: 13 September 2006
|
|
|
|
