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| Analysis of a Rice OsWRKY19 Gene Promoter |
| HAO Zhong-na;WANG Hai-hua;BAI Yang;GUO Ze-jian |
| Institute of Biotechnology,Zhejiang University,Hangzhou 310029,China |
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Abstract A 1404 bp promoter fragment of rice(Oryza sativa) transcription factor WRKY19 gene was amplified by PCR from rice Xiushui11 and named as OsW19p. The full length of OsW19p(p1404) and its 5' end deletions with a length of 105, 378, 9 77, 1 106, 1 205 and 1 306 bp were fused with gus gene, respectively. All of the contructed vectors were transformed into rice calli by Agrobacterium-mediated method. The results of histochemical GUS staining and fluorometric measurements showed: (1) Expression of gus gene was intensively inhibited by 2,4-D in the culture medium at callus stage; (2) Expression of gus gene was observed in roots, stems，leaves, flowers, and the embryos of germinating seeds. GUS staining were observed in adventititious, primary and lateral roots of rice seedlings, however, only lateral roots were stained at maturation phase; (3) GUS activity of all of deletions except p105 were detected in leaves of transformed plants, the level of p1205 was the highest, while expression was reduced for p977 and p1306. It suggests that some regulatory elements exist in -1404～-1306 bp and -1205～-977 bp, and fragments of -1306～-1205 bp and -977～-378 bp may contain negative elements.
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Received: 05 April 2005
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Corresponding Authors:
GUO Ze-jian
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| [1] |
ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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