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| Genetic Diversity and Molecular Markers of 5 Species of Snappers |
| LIU Li;LIU Chu-wu |
| Fisheries College , Guangdong Ocean University, Zhanjiang 524025, China |
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Abstract In order to protect and develop the valuable fishes of snappers, genetic diversity and molecular markers of 5 species of snappers, Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus，were detected using RAPD and SSR technique. The amplified products of genomic DNA in the 5 species of snappers by RAPD technique indicated that the polymorphic loci ratio (P ) were 89.30%，86.70%，92.11%,86.47% and 86.00%, the mean genetic distances of intra-species (D) were 0.3431，0.2130，0.3121, 0.1825 and 0.1775, and the genetic diversity indexes of intra-species (Hi ) were 0.1634，0.1095，0.1353, 0.1022 and 0.1024 respectively. Nine molecular markers, OPA8-413bp, OPA8-140bp, OPP10-418bp, OPA8-697bp, OPP10-526bp, OPA8-361bp, OPP10-449bp, OPA8-311bp and OPP10-599b , which could be used for identifying 5 species of snappers were found in the amplified products by primer of OPA8 and OPP10. The amplified products of genomic DNA in 5 species of snappers by SSR technique showed that effective numbers of allelic was 1.9610，3.3793，3.2957，1.7893 and 3.6591 respectively, average heterozygosities were 0.332，0.462，0.593，0.367 and 0.676, polymorphism information contents (PIC) were 0.302，0.438，0.554，0.332 and 0.641 respectively. Six molecular markers, 13 Prs229-115 bp, 4 Lca43-212 bp, 4 Lca43-240 bp, 13 Prs229-288 bp, 19 Prs275-156bp and 7Lca91-118 bp, which could be used for identifying 5 species of snappers were found in 11 loci of micrrosatellite.
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Received: 20 May 2005
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Corresponding Authors:
LIU Chu-wu
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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