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Abstract Abstract: As a useful tools, SSR markers are widely used in research of wheat genetics and breeding. SSR protocol included the DNA extraction, PCR reaction, electrophoresis in PAGE and staining. For a more economical, simple and precise protocol of wheat SSR analysis, this paper (1) compared three methods of DNA extraction from wheat seedling, seed and embryo; (2) compared the volume of PCR mixture; (3) compared three methods of PAGE staining. A more simple SSR protocol was set up as follows: the template DNA was obtained from embryo using simplified CTAB method; 10µL PCR mixture was used instead of 20 µL; the staining protocol was only three steps by combined fixing and staining to the first step and combined developing and stopping to the second step. The simplified SSR protocol saved 50% time and 50% money than before.
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Received: 16 October 2006
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