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Abstract Abstract: The object of this study is to screen the genes differentially expressed in flower induction by ethylene on bromeliads using suppression subtractive hybridization (SSH). SSH was performed in two directions to isolate the differentially expressed genes between exposed to ethylene for 1,6,24 h and the contract on Guzmania‘Attila’. The cDNA obtained from the final nested PCR were directly inserted into T/A cloning vector to establish subtractive hybridization library of specifically or highly expressed genes in flower induction by ethylene on bromeliads. The three forward SSH libraries contained 1063 clones. Reverse Northern dot blot was performed to screen the truly differentially expressed genes. Sequence analysis of these clones identified 990 differentially expressed clones, including 205 clones which were homologious to the reported genes in GenBank, and 313 clones were unknown genes. Information of this study could be used to isolate the Guzmania‘Attila’flower genes and understand the molecular mechanism of flowering.
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Received: 27 February 2009
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