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Abstract Abstract:The regulatory networks of the related genes at sow pregnancy stage was constructed by other in our laboratory, and some new and valuable candidate genes associated with sow pregnancy screened by bioinformatics analysis. A expressed sequence tag(EST) on the top of the networks was studied in the research. Primers were designed according to the sequence of the EST, the total RNA was extracted from sow ovaries，cDNA complete sequence cloned using RT-PCR and RACE-PCT was 596bp, the sequence was submitted and the accession number(FJ36413) was got from the GenBank database. The complete sequence included untranslated region( located in 1-67 and 422-596) and coding region(located in 65-421) coding a polypeptide of 117 amino acids. The predicted nucleotide sequence similarity between pig and human, mouse, and macaca mulatta were83%, 70%, 83%, amino acid sequence were 74%, 55%, 74%, respectively. The relative molecular weight of predicted DPPA5 was 13.60KD , and its isoelectric point was 5.24. The hydrophobic region located in 10-19, 24-42, 47-77, 84-90, and 101-118. The analysis result indicated that DPPA5 was an extrinsic protein.
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Received: 12 February 2009
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