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Abstract Abstract: Objective: To construct the recombinant plasmid pMAL-c4x -GnRH6 with gene fragment of the GnRH hexamer ,and express recombinant GnRH6 fusion protein in Escherichia coli TB1, and identification the fusion protein. Methods: The gene fragment of the GnRH hexamer t was connected to the pMAL-c4x plasmid, then recombinant pMAL-c4x -GnRH6 was induced in E.coli TB1under IPTG. SDS-PAGE, Western blot and animal experimentation was used to identify the expression product. Result: The molecular weight of fusion protein MBP-GnRH6 was about 50kD. And the reactivity of fusion protein and GnRH-antibody was better. Active immunization against MBP-GnRH6 resulted in the atrophy and degeneration of testis in male mouse. Conclusion: The recombinant plasmid pMAL-c4x -GnRH6 and engineering bacteria were successfully constructed and the recombinant MBP-GnRH6 fusion protein was verified with better reactionogenicity and immunogenicity, which can be used to immunocastrate in male mice.
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Received: 09 April 2009
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