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| Cloning and analysis of full-length LOX gene family cDNA |
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Abstract The conservative region of Lipoxygenase（LOX）gene from peach(Ruipan 5) was amplified from fruit cDNA, which was combined with the ESTs of LOX genes from GeneBank, and design the GSP primers after Blast aligning and sequence install. Three full-length different cDNA sequences were obtained, which were named PpLox1-3. The result of analysis indicated the three members of peach LOX gene family have high homogeneous with other plant LOX genes in protein level, but high heterogeneous in nucleotide level. Phylogenetic tree based on LOXs in Peach and other species indicated that PpLox1 was belonged to sort of 13-LOX and PpLox2-3 were belonged to sort of 9-LOX.
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Received: 15 December 2008
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