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Abstract Effects of EGCG, Cd2+ and EGCG+Cd2+ on the growth of PC-3 cells were investigated by MTT assay. Morphological changes of PC-3 cells were obtained with a inverted microscope. Apoptosis of PC-3 cells were measured according to Cell Apoptosis Rhodamine 123 detection Kit. Cell membrane fluidity was detected with Bruker ESR A300 spectrometer. EGCG and Cd2+ were screened for growth inhibition of PC-3 cells. And they were found to bring the changes of PC-3 cells morphologically. Apoptosis of PC-3 cells treated with 80 µmol/L EGCG were not induced. Meantime, cell necrosis was found by 20 µmol/L Cd2+. But apoptosis of PC-3 cells was induced by 80 µmol/L EGCG in the presence of 20 µmol/L Cd2+. Also, the membrane fluidity of PC-3 cells was decreased by EGCG and EGCG+Cd2+.
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Received: 07 November 2008
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