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| Detection for Pathogen of Bacterial Fruit Blotch of Watermelon by Immuno-capture PCR |
| Guan-Lin XIE |
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Abstract An immuno-capture PCR (IC-PCR) method for detection of Acidovorax avenae subsp. citrulli, a pathogen of bacterial fruit blotch of watermelon, has been established by combination of the ISE method with classical PCR and compared with the direct PCR method and growth-checking method. The results showed that all the strains of A. avenae subsp. citrulli tested produced 360bp specific fragments by the IC-PCR and direct PCR method, while others strains of 10 different genera showed negative PCR result. The minimum detection concentration for the IC-PCR and direct PCR method was about 50-102cfu/ml and 104cfu/ml, respectively. The sensitivity of the former is 100 times higher than that of later. Detection of 7 batches of different melon seeds from the markets by IC-PCR showed that 1 cantaloupe variety, 2 honey melon varieties and 2 watermelon varieties of seeds carried the pathogen which almost matched with the result of the growth-checking of the melon seeds. It indicates that the IC-PCR is accurate, sensitive, rapid and low cost.
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Received: 10 July 2006
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Corresponding Authors:
Guan-Lin XIE
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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