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| Effects of IGF-I, GH and CLA on the proliferation, differentiation and transcriptional factors mRNA expression of preadipocytes |
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Abstract To investigate the effects of IGF-I, GH and CLA on the proliferation and differentiation of preadipoctyes obtained from different depot sites. Our present study separated and cultured the preadipocytes from rat epididymis and abdominal subcutaneous adipose tissues. After diversity dose of IGF-I, GH and CLA treatments, cell proliferation and the extent of differentiation were detected by MTT method and oil-red O pigmentation respectively. The results showed that every dosage of IGF-I and CLA chould significantly promote both the proliferation and the extent of differentiation of the preadipocyte from each depot site, however, no significant change was observed after GH treatment. For the better understanding of the mechanism of IGF-I and CLA on the xubcutaneous preadipocytes, we also examed the mRNA expression levels of PPARγ and C/EBPα by semi-quantitative RT-PC method. It was found that 100ng/ml IGF-I markedly up-regulated the PPARγ and C/EBPα mRNA expression levels, while 48ng/ml GH and 100μM CLA could slightly increase that of PPARγ only, but no change of C/EBPα mRNA expression. This study suggested that IGF-I may stimulate the differentiation of rat preadipocyte primarily by enhancing the transcriptional factors mRNA expression, while the effect of CLA was mediated probably by up-regulating PPARγ mRNA expression, as well as activating of PPARγ as a ligand.
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Received: 05 December 2006
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