|
|
|
| The culture of transgenic tobacco with metallothionein gene from Tamarix. sp and its cadmium tolerance analysis |
| |
| |
|
|
|
|
Abstract A metallothionein gene from Tamarix. sp was directionally cloned into the pBI121 binary vector, in place of the XbaI – SacI GUS cassette. The Cauliflower mosaic virus (CaMV) 35S promoter/-nopalin synthase (nos) terminator system and kanamycin resistant gene (NPT II; neomycin phosphotransfers II) were used for these constitutive expression systems. The plasmid was then introduced into Agrobacterium tumefaciens (strain EHA105) by electroporation. Tobacco primary transformants were produced by leaf disc transformation. Kanamycin tolerance analysis showed that most transgenic plants have one copy of the exogenous gene in tobacco transformants. Southern and Northern hybridization indicates that exogenous gene has been integrated into the transgenic tobacco plants, and has been correctly expressed under the control of 35S promoter. Transgenic tobacco plants showed a better tolerant ability to cadmium than that of non- transformant.
|
|
Received: 02 June 2006
|
|
|
|
| [1] |
HUANG Xing, YAN Ai-Fen, DENG Ting-Xian, OUYANG Hong-Jia, LIU Lian, FENG Juan, ZHU Xiang-Xing, NIE Qing-Hua, TANG Dong-Sheng, ZHANG Xi-Quan. Construction of Zinc Finger Nuclease-induced Targeting Vector of Luchuan Pig (Sus scrofa) Fat1 Gene and Transgenic Study In vitro[J]. 农业生物技术学报, 2019, 27(8): 1369-1381. |
|
|
|
|
