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| Construct expression vector of Hepatitis B surface antigen gene and transformation to crowtoe |
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Abstract Abstract: Hepatitis B surface antigen gene was cloned from Hepatitis B virus DNA by using PCR reaction, then it was inserted into pBin438 and the plant expression vector pBHB was constructed. Transformed crowtoe by agrobacterium were analyzed for the integration of the transgene by PCR. It indicated the exterior gene had been integrated into the crowtoe.
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Received: 28 March 2006
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