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Abstract Two binary vectors containing tomato presystemin gene pNAR304（UbiI5’+Prosystemin+NOS3’）and pNAR305 (UbiI5’+Prosystemin+NOS3’ + PinⅡ5’+PinⅡ+PinⅡ3’) were constructed and were introduced into rice cvs. Xiushui63, Hejiang19 and Nipponbare by Agrobacterium-mediated transformation. From tests of hygromycin resistance, PCR and Southern blot, 14 independent transgenic plants were confirmed. Northern blot analysis indicated that both presystemin gene and PinⅡ gene could transcribed in these transgenic plants. But in the rice insect resistance bioassays to stripe stem borer (Chilo suppressalis) and brown plant hopper (Nilaparvata luguns stal), insect resistance of those plants transformed by prosystemin gene alone（pNAR304） showed no improvement than control plants and insect resistance of transgenic plants carrying both prosystemin gene and PinⅡgene（pNAR305）exhibited significant increase than control plants, but their insect resistance level was similar to those transgenic plants only could not regulate the expression of PinⅡgene in transgenic rice. From above experiment results, we think that there may be no signal pathway for tomato systemin in the rice and, probably, signal transmission of injury in rice were other pathway different from dicotyledonous systemin.
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Received: 28 March 2006
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