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| Analysis of the biochemical character, pathogenicity and antigenicity of the Staphylococcus aureus isolated from milk of dairy cows suffering from mastitis |
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Abstract Abstract: Fifty-three strains of Staphylococcus. aureus were isolated and identified from 93 milk samples of dairy cows suffering from mastitis, it’s the main pathogen in the three cow fields in Nanjing region. From the isolates six typical strains were selected to analysis with the biochemical characters and the inherited stability. Furthermore the other pathogenic factors of the six isolates were detected, such as coagulase, hemolysin and antimicrobial resistance factors etc. The crushed bacterial cells of 6 strains and 1 reference strain were processed with SDS-PAGE. The protein maps of the strains are similar except for the higher protein density of TQ-1. Mixture of whole bacterial cells of four S. aureus isolates(XL-1、TQ-1、XG-2 andT Q-2) were chosen to inoculate rabbit, and the hyperimmunized serum was used in the Western-blot assay to analyze the cross protective antigens of the isolates. The result shows the isolate XG-2 has unique band whose molecular mass is about 52KD in the NC membrane. According to the results, 2 S. aureus isolates were selected as the strains for preparing vaccine against bovine mastitis.
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Received: 30 March 2006
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