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Abstract Abstracts：A new modified two-temperature and multiple polymerase chain reaction (PCR) was developed for the simultaneous detection of WSSV( white spot syndrome virus) and TSV(taura syndrome virus ). Two sets of specific primers were designed based on the gene sequences of WSSV and TSV. It is shown that all samples which contained WSSV and TSV can be simultaneous amplified by the two-temperature and multiple polymerase chain reaction (PCR), but no specific band was amplified from control group of shrimp vibrio pathogen. The sensitivities for detection of WSSV and TSV are 10pg and 100pg respectively by this PCR. It was suggested that this two-temperature and multiple polymerase chain reaction (PCR) has high specificity and sensitivity.
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Received: 04 September 2006
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