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Abstract Intergeneric transfer of plasmid vectors pSET152 and pHL212 from Donor E. coli ET12567/pUZ8002 and S17-1 to Streptomyces cinnamonensis were demonstrated and optimized. NsdA gene disruption structure was constructed by PCR-targeting system and then introduced into Streptomyces cinnamonensis BIB2005 through intergeneric conjugal transfer.The disruption of nsdA was confirmed by PCR assays. Compared with the start strain, the yield of monensin of the nsdA mutant BIB309 increased 270 percent in the level of flask.
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Received: 03 April 2007
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