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| Screening and identification of proteins interacting with CASTOR by yeast two-hybrid system |
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Abstract CASTOR encoded predictable ion channel protein in Lotus japonicus ,which was the functional gene in the pathway of symbiosis, acted upstream of intracellular calcium spiking, mutants affected in CASTOR gene didn’t form root nodules. To research the proteins interacting with CASTOR, the CASTOR gene was used as the bait protein to mate with the cDNA lib of Lotus japonicus by yeast two-hybrid system. It had 111 positive clones in prescreening, it had 99 blue clones by thorough detection of β- Gal, there may be seven species proteins interacting with CASTOR after sequencing and NCBI blast analysis, then identified the expression of these genes in Lotus japonicus by RT-PCR, it was predicted that CASTOR may form compound with these proteins to thansfer symbiosis signal.
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Received: 20 March 2007
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